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subCULTIVATOR

The ultimate system for:

  • Adaptive Laboratory Evolution

  • Strain development

  • Microbiological adaptations and investigations

subcultivations in subCultivator

Subcultivations automated: impeccable timing and precision, unparalleled speed and reproducibility.

subCultivator - OD graph

how it  works

subCultivator - how it works

​A simple set up is all it takes to run a walk-away protocol with the subCultivator and admire the results.

A traditional series of flasks is replaced by a single mini bioreactor, connected to one outlet and three inlet syringe pumps. 

Subcultivation triggers can be defined as time, OD or fluorescence. For each subcultivation, media composition and process parameters can be defined, allowing fully automated strain development and microbial investigations. 

reactor

•    2 & 25 mL options
•    Multi-use, autoclavable 
•    Biofilm resistant, optically transparent
•    With breathable or sealed cap for aerobic and anaerobic cultivations
•    Magnetically stirred
•    4 ports (1 outlet, 3 inlets)
•    Glass mini bioreactors available

sensors

• Biomass (OD): accuRead, transmittance-based OD600 detection
• Fluorescence: fluoRead, 447 nm excitation laser combined with a spectrophotometer

fluidics

4 SYRINGE PUMPS FOR LIQUID HANDLING
•    1 outlet & 3 inlet high precision Essi Syringe pumps for media variations of subcultivation sequence
•    Compatible with 10 & 50 mL autoclavable syringes or single use 20 mL plastic syringes
•    Automatic syringe refilling solution available

applications

BIOPROCESSING
o    Batch, fed-batch and continuous
o    Chemostat and turbidistat


ADAPTIVE LABORATORY EVOLUTION
o Adaptive strain improvement by incremental increases of stressor concentration
o    Adaptations to process environment


MEDIA SCREENING
o    Automated growth & production investigations 

DEFINE TRIGGERS
o    Time
o    Biomass (OD)
o    Fluorescence
DEFINE PARAMETERS
o    Incremental increases in media composition
o    Import a table of bioprocess parameters including media compositions

control

ODdock software

features

widely applicable

  • Our detection systems work for all microorganisms, including filamentous ones

easy installation

  • All systems are safe and very easy to install in most shaking incubators

  • Systems are designed to allow autoclavability of culture vessels 

adaptable

  • All systems are suitable for aerobic and anaerobic cultivations

  • Vessel sizes are adaptable upon request

easy data handling

  • Data acquisition is handled by intuitive softwares

  • The saved data can be directly opened with Excel, Notepad etc.

Get in touch for more info and demos!

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