systems for real time OD acquisition on shaking incubators: get the most out of your cultivations
Real-time biomass monitoring is a valuable tool with diverse applications. It enables rapid strain screening, determination of minimal inhibitory concentrations for antimicrobial susceptibility testing, optimizing media composition for enhanced productivity in bioprocesses, monitoring cell growth in tissue engineering, assessing microbial activity in environmental studies and more. By providing real-time data on biomass levels, this approach facilitates quick decision-making, improves experimental throughput and reproducibility, as well as enhances overall efficiency across various scientific disciplines.
advantages of online biomass monitoring
-
faster decision making with less cultivations and effort
-
no manual sampling required
-
eliminated risk of contamination
-
more data points
-
greater reliability
-
availability of real time data
Our sensors are highly parallelizable, allowing significant improvements in experimental throughput.
OUR TECHNOLOGIES
Essi.tech is pioneering online biomass monitoring based on transmitted light detection, but our range of applications is extended with systems based on scattered light detection.
accuRead
transmitted light detection
scatterRead
scattered light detection
The light source and detector are facing each other. Biomass prevents the light from travelling through it; the higher the biomass, the lower the optical readout.
The light source and detector are next to each other. Biomass scatters light in all directions and some of it is bounced onto the light detector; higher biomass titers casue more light to be detected.
accuRead
transmitted light detection
The transmitted light detection principle of the accuRead technology is similar to spectrophotometers with two additions:
-
the detection principle is adapted to work online during the shaking operation
-
the dynamic LED power adaptation reassures readouts also in high biomass ranges
Because of this it is possible to measure a large range of biomass titers (ODs) during the shaking operation with the acquired data directly comparable to offline spectrophotometric measurements.
scatterRead
scattered light detection
The transmitted light detection principle of the scatterRead technology features an accelerometer to determine the optimal timeslot to take a measurement. Although the acquired data is not directly comparable to offline measurements with spectrophotometers, it is exceptionally simple to integrate and works with a large range of vessels.
comparison of technologies
*Occasional discrepancies between spectrophotometers as well as accuRead sensors are possible.
OUR SOFTWARE
OD dock software powers most of our biomass monitoring systems. It is easy to use, enables seamless parallelization and reliably acquires data that is easy to handle.
OUR SYSTEMS
flaskOD
Transmitted light-based OD monitoring system for Erlenmeyer flasks
FlaskOD system features custom Erlenmeyer flasks that allow transmitted light-based OD detection. accuRead technology allows accurate measurements during the shaking operation and our mounting solutions make highly parallelized experiments exceptionally simpe.
accuRead
transmitted light detection
scatterOD
Scattered light-based biomass monitoring system for a large range of vessels
The primary use of scatterOD is for vessels on shaking incubators that can not be customized for the integration of flaskOD sensor. A built in accelerometer reassures perfect measurements timing during shaking operation and our mounting solutions make workflow simple.
scatterRead
scattered light detection
mtpOD
Scattered light-based biomass monitoring system for 48 deep well microtiter plates
Our most high-throughput system represents a great leap forward in microbial studies. 48 experiments, each up to 5 mL in volume, can be executed on every shakerOD system, acquiring large amounts of data in the highest throughput form.
scatterRead
scattered light detection
ODfinger
Scattered or transmitted light-based biomass monitoring system using fiber optics
With the development of new approaches in microbial cultivations, new solutions for monitoring are required. ODfinger is our most versatile system that can reach any geometry and detect biomass in a huge range of volumes, from as low as only a couple of nanoliters to large scale, several cubic meter reactors.
scatterRead
scattered light detection
accuRead
transmitted light detection
FEATURES
widely applicable
-
Our detection systems work for all microorganisms, including filamentous ones
easy installation
-
All systems are safe and very easy to install in most shaking incubators
-
Systems are designed to allow autoclavability of culture vessels
adaptable
-
All systems are suitable for aerobic and anaerobic cultivations
-
Vessel sizes are adaptable upon request
easy data handling
-
Data acquisition is handled by intuitive softwares
-
The saved data can be directly opened with Excel, Notepad etc.